PyMol/ChimeraX

PyMol

  • select residues based on residue number
select nterm, resi 42+74+43+56+39
  • mutate one amino acid
cmd.wizard("mutagenesis")
cmd.fetch("target.pdb")
cmd.get_wizard().set_mode("LEU")
cmd.get_wizard().do_select("chain A and resid 22")
cmd.get_wizard().apply()
cmd.save("mutated_target.pdb")
  • save as .pdb file.
File > Export Structure > Export Molecule > Save > change "save as type"
  • select and color
select AFlow2, AF-A0A482RRZ8-F1-model_v6_aligned_CA and resi 57+147+148
color 0xFFDB13, AFlow2
  • diagram
    • Space-filling diagram:
      • shows all atoms
    • Ribbon/Cartoon diagram:
      • shows the protein backbone and highlights the alpha helices.
    • Surface diagram:
      • shows areas that are accessible to water molecules.

ChimeraX

  • color #1: 1305-1367 #00FE35